Poly-L-ornithine hydrobromide is a positively charged synthetic polyamino acid having one HBr per ornithine unit. It is a cristalline solid soluble in water. Applications for poly-L-ornithine hydrobromide are similar to poly-L-lysine hydrobromide and include the promotion of cell adhesion to solid substrates for culture dishes or slides, the conjugation to active molecules for improved activities, the layer-by-layer deposition techniques, and the complexation with nucleic acids for gene expression.
For cell culture applications, poly-L-ornithine hydrobromide with a molecular weight greater than 30,000 Da is recommended. The poly-L-ornithine hydrobromide (MW=49,000 Da) is easier to use because it is less viscous in solution; however, the poly-L-ornithine hydrobromide with an higher molecular weight (MW=78,000 Da) has more attachement sites per polymer chain available to the cells.
Our poly-L-ornithine hydrobromide has been purified by dialysis, sterile-filtered on 0.2um, lyophilized, and stored under Ar.
Store poly-L-ornithine hydrobromide under the condition of flushing with argon and protecting from light, and below -15°C.